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CRISPR RNA Knock-down
This technique offers a more specific and efficient alternative to traditional RNAi methods for knocking down RNA expression levels. CRISPR RNA knockdown using Cas13 provides a powerful and precise approach to regulate gene expression at the RNA level. Its ability to selectively degrade mRNA transcripts makes it a versatile tool for studying gene function and developing therapeutic strategies across diverse fields, including basic research, applied biotechnology, and medicine.
CRISPR RNA knockdown (KD) is particularly useful for studying gene roles by reducing RNA transcript levels. It can be applied in disease models, therapeutic development, RNA regulatory mechanisms, customized RNA therapies, antiviral applications, and high-throughput genetic screening.
Technical Background
Key Components:
- RfxCas13d (CasRx) Protein: A member of the CRISPR family that specifically targets RNA rather than DNA. RfxCas13d is derived from the bacterium Rhodococcus fascians.
- Guide RNA (gRNA): A synthetic RNA molecule designed to bind to the target mRNA. It contains a sequence complementary to the target RNA, guiding Cas13 to the specific mRNA for cleavage.
Features of Our Products
- Reduced Off-Target Effects: Compared to traditional RNA interference (RNAi), this method significantly minimizes off-target activity in cultured cells, offering greater specificity.
- Customizable Kits: We provide two distinct kits—vector kits designed for use with cancer cell lines and virus kits tailored for hard-to-transfect cells.
| Major Vector | Scramble Control Vector | |||||
|---|---|---|---|---|---|---|
| Class | Product Name | SKU | Information | Scramble Name | SKU | Information |
| Non-Viral All-In-one COT-P031k | CRISPR KN AIO Kit | TBD | The non-viral CRISPR kit includes three AI-designed gRNA sites tailored to target the customer-specified genes. Please click button to design the target genes. | Scramble Control CRISPR KN AIO Ctrl Kit, Vector type | TBD | The scramble sequence are used as non-specific cutting control |
| Viral All-in-one COM-P032k | CRISPR KN Lenti-AIO Kit | TBD | The CRISPR lentivirus kit packages three AI-designed gRNA sites into a single mixture tube for precise targeting and cutting of customer-specified genes. Please click button to design the target genes. | Scramble Control CRISPR KN Lenti-AIO Ctrl Kit, lentivirus type | TBD | The scramble sequence are used as non-specific cutting control |
Our design program assists customers in adjusting vector components and developing specific functions tailored to their unique requirements.
If you have any further inquiries regarding your project, please click the button to contact us. We are fully equipped to accommodate a wide range of your requirements.