Gene Knock-In Tagging
This technique provides versatile options for attaching selected tags to the 3′ or 5′ ends of customer-specified target genes, enabling precise tracking and functional analysis. Gene knock-in tagging can be applied across a broad range of research areas, including investigating protein localization, studying protein-protein interactions, analyzing gene function and regulation, creating transgenic models, and facilitating drug discovery efforts.
Technical Background
The CRISPR-Cas9 system is the core technology behind gene knock-in tagging. It utilizes a guide RNA (gRNA) to direct the Cas9 endonuclease to a specific genomic site, where it generates a double-strand break (DSB). This break is then repaired by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). For knock-in tagging, HDR is the preferred method, where a donor template containing the desired tag, flanked by homology arms, facilitates the precise insertion of the tag at the target locus
Key Components
- Cas9 protein: Creates a DSB at the target site.
- Guide RNA (gRNA): Directs Cas9 to the desired genomic locus.
- Donor template: Contains the tag (e.g., GFP, HA tag) along with homology arms to facilitate HDR-mediated knock-in.
Features of Our Products
- AI-Assisted Design: Our CRISPR and donor vectors are AI-assisted, designed to streamline and optimize the process for efficient target gene integration.
- User-Friendly Techniques: Our plasmid kits utilize lab-friendly methods that avoid the need for electroporation, making them easy to use in most labs. They are particularly well-suited for adherent cancer cell lines.
Our design program assists customers in adjusting vector components and developing specific functions tailored to their unique requirements.
If you have any further inquiries regarding your project, please click the button to contact us. We are fully equipped to accommodate a wide range of your requirements.
